Browsing by Author "Данильченко, С. І."

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ANGIOGENESIS, TISSUE HYPOXIA AND OXIDATIVE STRESS DURING MOTOR ACTIVITY OF VARIOUS DIRECTIONS AND INTENSITY
(2024) Gunina, L. M.; Orlov, A.; Danylchenko, S. I.; Golovashchenko, R.; Данильченко, С. І.
The study of the mechanisms of OS- induced effects with typical of it hypoxia-induced gene expression, is important in searching criteria for evaluation and prediction of physical efficiency. Recent studies have shown that oxygen-sensitive protein complex with transcriptional activity, namely hypoxia-induced factor (HIF-1α) plays the key role in the process of maintaining balance between the need for oxygen in living cells and its delivery. In mammals, HIF-1α is considered a leading inducer of transcription gene expression which responds to the lack of oxygen. HIF-1α is activated in physiologically important sites of oxygen pathway regulation, providing quick and adequate response to hypoxic stress, and, in particular, the expression of genes controlling the formation and growth of new blood vessels.
APPROACHES TO PHARMACOLOGICAL CORRECTION Of PSYCHOPHYSIOLOGICAL STRESS IN ATHLETES
(2021) Gunina, L. M.; Vysochina, N. L.; Danylchenko, S. I.; Mikhalyuk, E. L.; Voitenko, V. L.; Гунина, Л. М.; Высочина, Н. Л.; Данильченко, С. И.; Михалюк, Е. Л.; Войтенко, В. Л.; Данильченко, С. І.
The aim of the study was to assess the effectiveness of the in- fluence of the medical drug tivortin®aspartate (drinking solution of l-arginine aspartate) on the psychophysiological characteris- tics and the severity of psychophysiological stress in qualified athletes at the stage of direct preparation for the competition. Methodologically, the studies, which are randomized, blind, placebo-controlled, during the course (14 days) of drug usage in a daily dose of 40 ml were carried out in compliance with bio- ethical principles. the athletes in the control subgroups received placebo (3% glucose solution) in identical dosage, multiplicity and duration of usage. the assessment of the severity of psycho- physiological stress and its individual components was carried out in a technology modified by us according to the standard questionnaire of V.a. ivanchenko. the results obtained showed that in both control subgroups by the end of the observation period, although not too sig- nificant, but nevertheless, a significant increase in the value of the magnitude of psychophysiological stress was observed (from 38.08±1.38 to 41.67±1.71 points and from 37.52±1.24 to 39.94±0.99 points for athletes and weightlifters, respec- tively; in both cases changes are valid, p <0.05) and its indi- vidual components. On the contrary, in the athletes who used tivortin®aspartate in the dynamics of the study, the indicators of the severity of psychophysiological stress very significantly and reliably (from 48.67±0.69 to 39.17±1.07 in athletes and from 41.14±0, 46 to 39.97±0.79 in weightlifters; in both cases chang- es are valid, p <0.05) decreased, which indicated an improve- ment in psychological stability and a corresponding increase in mental stability to the forthcoming impact of competitive loads. In this way, the data obtained indicate an improvement in the mental readiness of athletes for the upcoming competitions and substantiate the advisability of using pharmacological substances based on l-argi- nine to improve psychophysiological characteristics during physical exertion with a different mechanism of energy supply. Целью исследования явилась оценка эффективности влияния препарата тивортин®аспартат (питьевой раствор L-аргинина аспартата) на психофизиологические характе- ристики и выраженность психофизиологического стресса у квалифицированных спортсменов на этапе непосредствен- ной подготовки к соревнованиям. Методологические исследования, по дизайну представ- ляющие собой рандомизированные слепые плацебо-кон- тролируемые, в динамике курсового (14 дней) применения препарата в суточной дозе 40 мл проводили с соблюдением биоэтических принципов. Плацебо (3% раствор глюкозы) спортсмены в контрольных подгруппах получали в иден- тичной дозировке, кратности и длительности применения. Оценку выраженности психофизиологического стресса и его отдельных компонентов проводили по модифициро- ванной авторами технологии по стандартному вопроснику В.А. Иванченко. Полученные результаты показали, что в обеих контроль- ных подгруппах к концу периода наблюдения выявлено хотя и не слишком значительное, однако достоверное увеличе- ние значения величины психофизиологического стресса (c 38,08±1,38 до 41,67±1,71 баллов и с 37,52±1,24 до 39,94±0,99 балла у легкоатлетов и тяжелоатлетов, соответственно) и его отдельных компонентов, а у спортсменов, применяв- ших в динамике исследования тивортин®аспартат, показа- тели выраженности психофизиологического стресса весь- ма значительно и достоверно снижались с 48,67±0,69 до 39,17±1,07 у представителей легкой атлетики и с 41,14±0,46 до 39,97±0,79 – у тяжелоатлетов, что указывает на улучше- ние психологической стабильности и соответствующее по- вышение психической устойчивости к предстоящему воз- действию соревновательных нагрузок. Таким образом, полученные данные указывают на улуч- шение психологической готовности спортсменов к пред- стоящим соревнованиям и обосновывают целесообразность применения фармакологических субстанций на основе L-аргинина для улучшения психофизиологических характеристик при физических нагрузках с разным механизмом энергообеспечения.
AROMATHERAPY IN THE TREATMENT OF SEASONAL RESPIRATORY VIRAL INFECTIONS
(2023) Yashchyshyn, Z. M.; Horoshko, V. I.; Danylchenko, S. I.; Ящишин, З. М.; Горошко, В. І.; Данильченко, С. І.
To determine the quality of the oil, it is necessary to conduct a number of complex laboratory studies, including a gas chromatographic analysis of the composition. This test is costly and will often not be carried out by users of aroma oils (private patients, doctors, rehabilitators, etc.). Although there is such equipment for diagnosing the charac- teristics of the oil, there is no standard database against which to compare the composition of the oil. The aim of this trial is to inform practitioners and consumers about the rules to be observed when working with EO and possible side effects with the help of modern gadgets. Methods. To achieve the set goals and solve certain problems, the following research methods were used, in particular: problem-chronological - to establish the chronology of the scientific study of the use of aromatherapy; socio- logical - a study of the medical and social qualities of the spread of pathological changes in the autonomic nervous system; analytical - to determine scientific guidelines, the use of evidence-based medicine in aromatherapy procedures; the method of terminological analysis, which made it possible to ensure the disclosure of the essence of the phenomena under study; the method of scientific extrapolation, which made it possible to determine the possibilities of creating and applying aromatherapy as an optimal synthesis of theoretical and practical knowledge; methods of multivariate statistical analysis and forecasting for data processing. Project objectives: 1. Develop a classification of EM, taking into account the standardization of requirements for them. 2. To study data on the effect of aerosol therapy with natural essential oils on the microbial and viral background of the body. 3. Analyze changes in cellular and humoral immunity before and after aerosol therapy with natural essential oils. 4. Develop a methodology and algorithm for conducting aerosol therapy with natural essential oils, and create a database for EO. 5. To study the clinical effectiveness of aerosol therapy with natural essential oils in complex rehabilitation. Results. The developed database will make it easy to navigate a huge variety of proposed types of essential oils and will allow you to establish its correspondence with the producing plant, which, unfortunately, cannot always be observed in essential oils offered for sale. Moreover, the aromatic oils included in the base must be checked for toxicity and correct use. Misuse may result in side effects, including allergic reactions, narcotic, carcinogenic or neurotoxic ef- fects. This database is created with the possibility of constant updating and adding new information in all its forms, is the initial stage of creating a more complete version and can be claimed by interested organizations, and individuals via the Internet. Conclusions. It is advisable to use phytoaeroprophylaxis in medical institutions (hospitals, medical units, health centers, sanatoriums, maternity hospitals, nursing homes, etc.). Of course, a return to natural remedies, in particular to aerophytotherapy, and phytoaeroprophylaxis, should be combined with the study of their action at the modern methodo- logical level, thorough clinical development of indications and contraindications for their use. Сучасна ароматерапія – профілактичний, оздоровчий, повністю природний спосіб, який ефективно застосовується в практиці фізичної реабілітації. Мета. Поінформувати практиків і споживачів про правила роботи з ефірними оліями та можливі побічні ефекти за допомогою сучасних гаджетів. Матеріали і методи. У роботі використані методи дослідження: проблемно-хронологічний – встановити хронологію наукового вивчення використання аромотерапії; соціологічний – дослідження медико-соціальних якостей поширення патологічних змін у вегетативній нервовій системі; аналітичний – для визначення наукових орієнтирів, застосування доказової медицини в процедурах ароматерапії; метод термінологічного аналізу – для розкриття сутності досліджуваних явищ; метод наукової екстраполяції, що дав змогу визначити можливості створення та застосування ароматерапії як оптимального синтезу теоретичних і практичних знань; методи багатофакторного статистичного аналізу. Результати. Розроблена база даних дозволить легко зорієнтуватися у величезній різноманітності запропонованих видів ефірних олій і дозволить встановити їх відповідність заводу-виробнику. Ароматичні масла, що входять до складу бази, повинні бути перевірені на токсичність і правильність використання. Ця база даних створена з можливістю постійного оновлення та додавання нової інформації у всіх її формах, є початковим етапом створення більш повної версії та може бути затребувана зацікавленими організаціями та окремими особами через Інтернет. Висновки. Запропонована база даних дозволить легко орієнтуватися у величезній різноманітності пропонованих типів ефірних масел і дасть можливість користувачам встановлювати його відповідність із за- водом-виробником.
ASSESSMENT OF MEDICAL ERRORS IN DENTISTRY
(2023) Babkina, O.; Danylchenko, S.; Данильченко, С. І.
The purpose of the work was to analyze the situation with the provision of dental services by medical staff of dental clinics and to analyze medical records to establish/refute the presence of defects in the provision of services. Results. As a result of the analysis of 56 cases of dental services, it was found that surgical dental services (implantation) in most cases are provided in accordance with generally accepted recommendations. In most cases, medical records in dental clinics are properly drawn up, which is of great importance in its study and analysis of the presence/absence of defects during dental manipulations at the time of forensic medical examinations. However, in 10% the medical records of the dental patient were improperly issued, the dates of admission were not specified, the scope of the manipulations performed was not described, the review of the control examination was not scheduled, the necessary instrumental methods of examination were not appointed, the recommendations for cavity care were not provided, etc. Conclusion. The problem of providing services of inadequate quality and improper performance of the professional duties by dental clinics staff, careless and dishonest attitude to their professional duties remains relevant. Medical records data of dental clinics allow experts to analyze the nature, scope, stages and completeness of the provision of dental services, identify/refute the presence of defects in the proper/improper provision of surgical services (implantation) by dental clinics staff, establish/refute a causal relationship between the actions of dental clinics staff and consequences in patients.
CASES OF DOMESTIC VIOLENCE - DOCTOR'S ACTIONS AND DOCUMENTING THEM. EXPERIENCE OF UKRAINE
(2024) Babkina, O. P.; Danylchenko, S. I.; Horoshko, V. I.; Данильченко, С. І.
At the legislative level in Ukraine, rights, obligations, benefits for citizens are provided for, normative documents have been developed on the prevention and counteraction of violence, protection and observance of human rights. According to Article 3 of the Constitution of Ukraine, a person, his life and health, honor and dignity, inviolability and security are recognized as the highest social value in Ukraine, human rights and freedoms and their guarantees determine the content and direction of the state's activities. Ensuring human rights and freedoms is the main duty of the state. The purpose article is an analysis and generalization of recommendations regarding the algorithm of actions of medical workers when documenting cases of domestic violence (physical, sexual, psychological, economic) and providing medical and psychological assistance to victims while respecting the rights of patients and ensuring their safety.
COMPLEX REHABILITATION OF ISCHEMIC HEART DISEASE PATIENTS AFTER CORONARY BYPASS OPERATION WITH THE USE OF BIOLOGICALLY ACTIVE ADDITIVES
(2023) Horoshko, V. I.; Yashchyshyn, Z. M.; Danylchenko, S. I.; Morozenko, D. V.; Gliebova, K. V.; Kononenko, N. M.; Yeromenko, R. F.; Данильченко, С. І.
Aim: The purpose of the work was to study the possibility of using Dihydroquercetin in rehabilitation programs for patients with coronary artery disease after Coronary artery bypass grafting (CABG) surgery in order to increase the effectiveness of rehabilitation, which has notable scientific and practical significance. Materials and Methods: The methodological basis of the research is based on systemic, historical, terminological, and informational approaches. Results: The results of the study showed the high clinical effectiveness of Dihydroquercetin in the complex medical rehabilitation of patients with coronary artery disease who underwent the operation. The pill intake did not affect indicators of carbohydrate and lipid metabolism, which indicates its metabolic neutrality. None of the patients had any worsening of the condition, increased frequency of angina attacks, or episodes of painless ischemia. All patients indicated good tolerability of the drug, and improvement in general well-being (reduction or disappearance of shortness of breath, decrease in pain intensity, increase in activity, improvement in sleep). The implementation of the optimized rehabilitation program also contributed to the improvement of the psycho- emotional state of the patients. Conclusions: Сomplex medical rehabilitation of patients with coronary heart disease after Coronary artery bypass grafting surgery according to a program with the inclusion of Dihydroquercetin helps to improve the rheological properties of blood, strengthen the active vasomotor mechanisms of MC regulation, improve indicators of central and peripheral hemodynamics, blood oxygenation, increase in exercise tolerance, improve psycho-emotional the condition, which ultimately ensure an increase in the rehabilitation effect.
CRANIOTOPOGRAPHIC CHARACTERISTICS OF SINUOUS-LIQUOR RELATIONSHIPS IN MATURE PEOPLE
(2023) Bondarenko, S. V.; Dubina, S. O.; Serbin, S. I.; Khapchenkova, D. S.; Fedorova, I. O.; Koptev, M. M.; Danylchenko, S. I.; Бондаренко, С. В.; Дубина, С. О.; Сербін, С. І.; Хапченкова, Д. С.; Федорова, І. О.; Коптев, М. М.; Данильченко, С. І.
The purpose of the study was to determine the craniotopographic (craniometric) indicators of sin- uous-liquor relationships in the horizontal plane in people of the first and second periods of mature age, based on the maximum permissible depth of its intro- duction. Materials and methods. The object of the study was the dura mater of the brain with its sinuses, the ventricles of the brain and the vault of the skull of ma- ture people. The study was performed on 100 corpses of peo- ple of different ages and gender with the manufac- turing of native preparations of the brain with mem- branes and liquor structures. Of the indicated number of morphological objects, 70 preparations of veins and sinuses of the dura mater of the brain and 30 prepara- tions of the cerebrospinal fluid system of the brain of mature people were made. The study used such research methods as: mac- ro- and micropreparation of anatomical objects of the brain; cranio- and morphometry of veins, sinuses of the dura mater of the brain and liquor structures of the brain; manufacturing of corrosive (acrylic) casts – preparations of veins, sinuses of the dura mater of the brain and liquor formations of the brain; injection tech- nique; variational-statistical analysis of morphometric data; computer-graphic analysis. Results and discussion. As a result of the study, it was found that the puncture of the central part of the lateral ventricle is better to be performed at the level of the bregma point, or between this point and the vertex (v). The puncture needle penetrates to a depth of 6.0–7.0 cm through the milling hole, which is displaced by 3.0–4.0 cm from the arrow line at an angle of 70–75°. The obtained data make it possible to determine the most optimal craniometric puncture points of the cerebrospinal fluid system of the brain. To penetrate into the cavity of the anterior horn of the lateral ventri- cle, the metopion point is proposed, which is located at the intersection of the median plane of the head with the line connecting the most convex areas of the frontal humps. The upper puncture of the lateral ventricle is better to be performed by puncturing at the bregma point (the point of connection of the sagittal and cor- onal sutures) 2.0 cm from the midline (sagittal plane); the needle is directed down and backwards and at a depth of 5–6 cm (taking into account that it is enlarged and stretched) it enters the ventricular cavity. The suboccipital puncture or a puncture of the cerebellar-cerebral cistern must be performed be- tween the opisthocranion and lambda points. The puncture needle is inserted to a depth of 6.0–7.5 cm, and the end of the needle is gradually lowered to the base of the skull. Conclusion. The most optimal puncture points of the constituent parts of the cerebrospinal fluid system of the brain – the cavity of the anterior horn and the central part of the lateral ventricle, the cavity of the cerebellar-cerebral cistern – were determined using craniometric analysis. Метою дослідження було визначення у людей першого і другого періодів зрілого віку кра- ніотопографічних (краніометричних) показників пазушно-лікворних взаємовідношень у горизонтальній площині. Матеріал та методи. Об’єктом дослідження були тверда оболона головного мозку з її пазухами, шлуночки головного мозку та склепіння черепа людей зрілого віку. У дослідженні застосовані методи макро- та мікропрепарування анатомічних об’єктів головного мозку; краніо- та морфометрія вен, пазух твердої оболони головного мозку та лікворних структур головного мозку; виготовлення корозійних (акрилових) зліпків – препаратів вен, пазух твердої оболони головного мозку та лікворних утворень головного мозку; ін’єкційна методика; варіаційно-статистичний аналіз морфометричних даних; комп’ютерно-графічний аналіз. Результати дослідження. У результаті дослідження встановлено, що пункцію центральної час- тини бічного шлуночка краще проводити на рівні точки брегма, чи між цією точкою та вертексом (v). Пункційна голка проникає на глибину 6,0–7,0 см через фрезовий отвір, який зміщений на 3,0–4,0 см від стрілової лінії під кутом 70-75°. Верхню пункцію бічного шлуночка краще здійснювати проколом у точці bregma (точка з’єднання стрілового та вінцевого швів) на 2,0 см від серединної лінії (стрілової площини); голку спрямовують донизу та дозаду і на глибині 5–6 см потрапляють у порожнину шлуночка (враховуючи, що вона збільшена та розтягнута). Субокципітальну пункцію або пункцію мозочково-мозкової цистерни необхідно проводити між точками опістокраніон та точкою ламбда. Пункційна голка проводиться на глибину 6,0-7,5 см, причому, з поступовим опусканням кінцевої частини голки до основи черепа. Висновок. Визначені за допомогою краніометричного аналізу найбільш оптимальні точки пункції складових частин лікворної системи головного мозку – порожнини переднього рогу і центральної частини бічного шлуночка, порожнини мозочково-мозкової цистерни.
CRANIOTOPOGRAPHIC FEATURES OF THE TRANSVERSE SINUSES OF THE DURA MATER OF THE BRAIN OF THE CRANIAL VAULT OF MATURE PEOPLE
(2024) Bondarenko, S. V.; Serbin, S. I.; Dubyna, S. O.; Kalyuzhna, V. V.; Bilash, V. P.; Svintsytsʹka, N. L.; Danylchenko, S. I.; Бондаренко, С. В.; Сербін, С. І.; Дубина, С. О.; Калюжна, В. В.; Білаш, В. П.; Свінцицька, Н. Л.; Данильченко, С. І.
The goal of our research is the description of craniotopographic features of the transverse sinuses of the dura mater. The aim and the methods of research. In order to achieve the goal, a set of methods for morphological studies was developed: macro- and micropreparation of anatomical objects of the brain; cranio– the morphometry of veins, sinuses, and the cervian structures of the cephalic brain; preparation of corrosive (acrylic) ulcerative preparations of veins, sinuses of the dura mater and pervoric lesions of the head; injection technique; variational and statistical analysis of morphometric data; computer-graphic analysis. This study was conducted on 70 preparations of veins and sinuses of the dura mater of the brain. Results of the study and their discussion. Considering the above-mentioned facts, we established a craniotopographical projection of the transverse sinuses in relation to generally accepted craniotopographical planes and points. According to the data we have obtained, the venous collectors (transverse sinuses) studied by us have a certain projection analogy on the bones of the cranial vault and the corresponding correlations to the craniometric points, which can be used in medical craniology. In this regard, there is a natural range of individual anatomical variability in the parameters of the dura mater, which are described in people of mature age. The obtained data on the structure, shape, size and position of the venous collectors of the cranial vault (transverse sinuses) can be used in surgical interventions on the cerebrospinal fluid system, including the ventricles and cerebrospinal fluid pathways. Transverse sinuses, located on both sides of the skull in the horizontal plane, are of important practical importance from a neurosurgical point of view. In adults, fully formed right and left transverse sinuses are always noted, which perform an important transport function of carrying venous blood from the drain of the sinuses into the sigmoid sinus and internal jugular veins. The transverse sinuses of the posterolateral surface are adjacent to the inner surface of the parietal and occipital bones, which explains the frequent damage and injury to the collector in various skull injuries. In addition, these collectors must be taken into account during surgical access to various parts of the brain, including the lateral ventricles. Taking into account the above, it is possible to distinguish different areas of the accumulation of veins that flow into the transverse sinuses. The densest location of tributaries, branches and anastomoses is observed in the initial part of the collectors, near the drainage of the sinuses, due to the surface veins of the occipital lobe and the veins of the cerebellar tent. The second place of accumulation of venous vessels is noted in the area of the transition of the transverse sinuses into the sigmoid sinuses, where the superficial temporal and parietal veins are concentrated. Conclusions. The obtained results not only complement the known facts, but also more deeply reveal the prin- ciples of the craniotopographic structure of the transverse sinuses of the dura mater of the cranial vault of people of mature age. On the basis of the actual data obtained, the position is substantiated, according to which a kind of “vascular zone” in the middle part of the transverse sinuses is topographically distinguished, where, in our opinion, it is better to perform surgical interventions in neurosurgical practice. Метою дослідження було встановлення краніотопографічних особливостей поперечних пазух твердої оболони головного мозку склепіння голови (черепа) людей зрілого віку. Матеріал та методи. Дослідження проведено на 70 препаратах вен і пазух твердої оболони головного мозку. За результатами аналізу уточнено топографо-анатомічні положення поперечних пазух дорослої людини; встановлено краніотопографічну проєкцію поперечних пазух стосовно загальноприйнятих краніотопографіних площин і точок; з’ясовано індивідуальну анатомічну мінливість поперечних пазух людини зрілого віку з різними типами будови голови (черепа) за такими досліджуваними ознаками, як: дов- жина, ширина верхньої стінки, ширина задньої стінки, ширина нижньої стінки та висота просвіту; уточнена відома наявність венозних притоків й анастомозів упродовж поперечних пазух. Результати. Встановлено, що вищеперелічені венозні колектори мають певну проєкційну аналогію на кістки склепіння черепа та відповідні співвідношення до краніометричних точок, які можуть бути використані в медичній краніології з урахуванням природного діапазону індивідуальної анатомічної мінливості параметрів твердої оболони головного мозку, котрі описано в людей зрілого віку. Висновки. Встановлено різні ділянки скупчення вен, які впадають у поперечні пазухи. Найбільш густе розташування притоків, гілок і анастомозів можна спостерігати в початковому відділі колекторів, поблизу стоку пазух завдяки поверхневим венам потиличної частки та венам намету мозочка. Друге місце скупчення венозних судин відзначаємо в ділянці переходу поперечних пазух у сигмоподібні, де сконцентровані поверхневі скроневі та тім’яні вени. Тож коли ґрунтуємося на встановленій топографії виділяємо своєрідну «безсудинну зону» в середній частині поперечних пазух, яка є благодатною при виконанні оперативних втручань на венах і синусах твердої мозкової оболони й лікворній системі головного мозку.
DIAGNOSIS OF BLUNT TRAUMA OF KIDNEY INJURY WITH INFRARED THERMOMETER METHOD
(2020) Babkina, O. P.; Danylchenko, S. I.; Varukha, K. V.; Volobuev, O. E.; Ushko, Ia. A; Бабкина, Е. П.; Данильченко, С. И.; Варуха, Е. В.; Волобуев, А. Е.; Ушко, Я. А.; Данильченко, С. І.
Aim - the study of the prescription of injury on the dynamics of changes in temperature indices of the injured kidney tissues in blunt trauma. The material of the study to date are the tissues of the kidneys of 256 male and female persons, aged 20 to 60 years, who died at a known time of trauma and prescription of death in the were subject to autopsy. To address the question of the age of occurrence of kidney damage, we used a modern method of infrared thermom- etry (using a TH 9100 PMVI-WL Thermo Tracker thermal imager, which is a contactless high-sensitivity infrared camera). In this way, we came to the gown, who, when injured by blunt objects, are willing to take into account the laws of the organs of the black empty. However, in the area of trauma throughout the period after the autopsy of the dead (24 hours of study), observed higher quantitative temperature compared with non-injured kidney tissues, on average, 2-3 ° C (± 0.28). To take into account the fact that the right kidney is protected more than the left kidney and less vulner- able to body compression, it is not accompanied by a shift in the impact of traumatic objects; at 1.5-2 ° C (± 0.14). We believe that determining the limitation period of the onset of mechanical trauma by changes in the infrared thermometry indices carried out in the complex are appropriate and such that provide the full amount of information about the time of causing the trauma. Цель исследования - изучение влияния травмы на дина- мику изменения температурных показателей поврежденных тканей почек при тупой травме. Материалом исследования служили ткани почек, забранные во время вскрытия у 256 мужчин и женщин в возрасте от 20 до 60 лет, умерших от травмы, с известным временем давности наступления травмы и смерти. Для решения вопроса о време- ни повреждения почек применен современный метод инфра- красной термометрии с использованием тепловизора TH 9100 PMVI-WL Thermo Tracker, (NEC Avio infrared Technologies Co. Ltd., Япония), который является бесконтактной высокочув- ствительной инфракрасной камерой. В результате проведенного иссследования установлена характерная динамика изменения температурных показа- телей при травме почек тупым предметом. Однако, в зоне травмы в течение всего периода после вскрытия (24 часа исследования) наблюдались более высокие количественные температурные показатели непосредственно в области трав- мы почки, в среднем, на 2-3°С (±0,28) в сравнении с непо- врежденными тканями почки. Температурные показатели правой почки снижаются медленнее, в среднем, на 1,5-2°С (±0,14) относительно левой, что связано с топографоанато- мическими особенностями расположения почек. Установлено, что при тупой травме в поврежденных и ин- тактных тканях почек наблюдается закономерная динамика уменьшения температурных показателей, что может быть использовано для определения времени наступления трав- мы с помощью метода инфракрасной термометрии.
DIAGNOSTICS OF THE PRESCRIPTION OF DAMAGE TO THE PANCREAS IN EXPLOSIVE TRAUMA AND GUNSHOT WOUNDS DURING A MILITARY CONFLICT
(2022) Babkina, O.; Danylchenko, S.; Данильченко, С. І.
Introduction: Quite often a pancreatic injury is observed in combi- nation with the injury of other organs of the abdominal cavity during explosive trauma and gunshot wounds during a military conflict. Purpose: To study the dynamic changes of histological parameters injured pancreas tissues in various types, depending on the period of injury. Materials and methods: The material for the study included tissues of 52 male and female pancreases, aged from 20 to 54 who died of injury of explosive trauma and gunshot wounds during a military conflict. In the conducted research we used histological and histochemical techniques.
DYNAMICS OF BIOCHEMICAL MARKERS OF THE CONNECTIVE TISSUE METABOLISM IN THE BLOOD OF RATS AFTER INSERTION OF STEEL IMPLANTS WITH DIAMOND-LIKE CARBON COATING INTO THE FEMUR
(2021) Makarov, V.; Morozenko, D.; Gliebova, K.; Danylchenko, S.; Данильченко, С. І.
Objective: It is known that the regeneration of bone tissue after implantation of any structures depends on the coating of their surface. Therefore, the research of the influence of steel implants with diamond-like carbon coating on regeneration of bone tissue in the dynamics after implantation is a topical issue. Material and Methods: The study was conducted on 61 male rats, 5 of them were intact animals. There were also 2 groups of rats with 28 animals in each group (the 1 st group was the control one where implants were without diamond-like carbon coating, the 2 nd group was experimental where the implants were without coating). We determined the content of glycoproteins, chondroitin sulfates, alkaline phosphatase, total calcium, oxyproline and osteocalcin in the blood of rats on the 7th , 14th, 30th and 90th days after implantation. Results and Discussion: Group 2 rats had a faster osteointegration compared with the group 1 rats, which was manifested by the normalization of biochemical markers of bone metabolism (glycoproteins, chondroitinsulfats, oxyproline and osteocalcin) on the 90th day of an experiment. Conclusion: Using steel implants with diamond-like carbon coatings showed that the content of glycoproteins and chondroitin sulfates increased only on the 7th day; oxyproline and osteocalcin increased on the 7th , 14th and 30th days after implantation; on the 90th day the rates of these biochemical markers reached the norm, indicating a high efficiency of osteointegration.
ENERGY PRODUCTING AND APOPTOSIS AT PHYSICAL LOADS: THE ROLE OF THE CHANGES THE PROOXIDANT-ANTIOXIDANT BALANCE (A REVIEW)
(2020) Voitenko, V.; Gunina, L.; Danylchenko, S.; Nosach, O.; Данильченко, С. І.
The article notes that the development of oxidative stress and the violation of cellular energy balance is the primary link of the vast majority of systemically-forming homeostatic shifts in the athlete’s body and changes the vital structure and function of cellular and subcellular proteins membrane. Changes in the quantitative and qualitative composition of lipid components of membranes, inhibition of the activity of key glycolysis enzymes, as well as the deterioration of bioenergy mechanisms, result from the accumulation of free radicals due to activation of lipid peroxidation. The protection of the organelles responsible for energy supply from oxidative effects is provided by mitochondrial disconnecting proteins that exist in the myocardium. The development of metabolic ischemia due to the imbalance between the delivery of oxygen to cardiomyocytes and their need for myocardium is accompanied firstly by the inhibition of the process of oxidation of glucose and an increase in the use of fatty acids, and then the accumulation of lactate with the development of acidosis of the intracellular environment and the impairment of the ability of myocytes and cardiomyocytes to relaxtion and contraction. It has been established that strenuous muscle activity leads to the formation of a hypoxic state with its characteristic redistribution and increase of energy, metabolic, structural resources of the body in the interests of the tissue where adaptive adjustments are taking place. The insufficiency of energy generation due to the development of this state leads to the dysfunction of the mitochondrial apparatus, which subsequently causes the violation of the energy supply, antioxidant protection, membrane stability due to intensification of lipid peroxidation and leads to cell apoptosis. This forms a background for the occurrence of fatigue and tension, followed by reduction of physical performance of athletes. The detection of the above changes makes it possible to prevent and correct in a timely manner the negative effects of oxidative stress associated with ultra-intensive physical loads.
EXPERIENCE OF DETERMINING THE PRIORITY OF COMPLEX PROCESS OR SYSTEM (ON THE EXAMPLE OF PHYSICAL EDUCATION AND SPORT)
(2020) Olkhovyі, O.; Chernozub, A.; Danylchenko, S.; Korchagin, М.; Romanchuk, S.; Oderov, A.; Klymovych, V.; Zhembrovskyi, S.; Данильченко, С. І.
The most used in today's methods of expert assessments (questionnaires, surveys, brainstorming) have a number of underestimations, the main of which is that the consistency and logic of expert statements are not determined. To solve this problem field, a computer program has been created for collecting, saving, quantitative comparison and operational processing of the results obtained through a collective expert evaluation. The article describes the mathematical algorithm of this program. As a base of the program, due to the combination of elements of the Delphi expert survey and the method of modern theory of hierarchical systems T. Saati, the procedure of synthesis of priorities is taken, which is calculated on the basis of subjective expert estimation. The priority or weighting factor is determined on the basis of the individual judgment pair comparison principle of the expert on the priority (the degree of advantage, weight) of one component of a particular process over another. In this work, the method of simple iteration is used. The created program establishes the judgments coherence and logic degree of each of the experts. That is, by way of the estimation pair comparison of the individual expert it is not permissible to violate the coherence and logic of his estimations. A dual comparison excludes the possibility of "blind" use of an uncoordinated and illogical evaluation, requires its refinement or ignores the use of such an assessment. With the help of convenient and easy to use author's computer program, scientists were able to quickly obtain quantitative units of weighting factors of the studied components of a particular process. This allows to display all the results of an interdependent collective, logical and coordinated individual expert estimation.
IMPROVING THE TRAINING OF QUALIFIED ATHLETES-STUDENTS BY MODERN NUTRITIOLOGICAL TECHNOLOGIES
(2021) Gunina, L. M.; Chernozub, A. A.; Danylchenko, S. I.; Voitenko, V. L.; Milashius, K. M.; Гуніна, Л. М.; Мілашюс, К. М.; Чернозуб, А. А.; Данильченко, С. І.; Войтенко, В. Л.
Currently, a significant part of highly qualified ath- letes are students of higher educational institutions at the same time, which manifests itself not only in an ul- tra-high level of physical activity, but also in significant psycho-emotional stress. Therefore, the search and introduction of modern technologies in the process of sports training, including pharmacological, hygienic, rehabilitation, etc. are continuing. An essential place among such technologies is occupied by a compre- hensive methodology of nutrition-metabolic support of the training process, built on the principles of ev- idence-based medicine and using the latest achieve- ments of laboratory and functional diagnostics, bio- chemistry and pharmacology. The purpose of the study is the formation of ideas about the necessity, validity and effectiveness of nutri- tive-metabolic support of the process of sports train- ing of students of higher education institutions. Nutrition-metabolic support is part of a sporting nutritiology that possesses all the features of basic science – terms, definitions, and at the same time has great practical significance, helping to form rel- evant and well-grounded programs of nutrition-meta- bolic support of the training and competitive process of athletes, as well as to contribute to preservation the health and quality of life of athletes. This position is particularly important in student sports, where the body of an athlete is exposed to additional stressors due to the complexity of the need for a rational and effective combination of the educational and training process of highly qualified athletes. Conclusion. The applied nutritive-metabolic tech- nologies such as methods of metabolic support of mo- tor activity should take into account the specialization and qualifications of athletes, their gender-age char- acteristics and should be applied depending on the training period and the orientation of the loads. In con- nection with the improvement and tightening of dop- ing control, it is extremely important that sports nutri- tion products and special nutritional supplements that are widely used to optimize the functional state of the athlete’s body and maintain their health and quality of life do not contain substances related to the World Anti-Doping Agency Prohibited List, while ensuring a pronounced ergogenic effect. В даний час значна частина висококваліфікованих спортсменів є одночасно студентами вищих навчальних закладів, що проявляється не тільки у надвисокому рівні фізичних навантаженнях, але і значному психоемоційному напруженні. Тому пошуки й впровадження в процес спортивної підготовки сучасних технологій, включаючи фармакологічні, гігієнічні, реабілітаційні та ін., постійно тривають. Вагоме місце серед таких технологій займає комплексна методологія нутритивно-метаболічної підтримки тренувального процесу, яка побудована на принципах доказової медицини, і використовує найостан- ніші досягнення лабораторної та функціональної діагностики, біохімії і фармакології. Нутритивно-метаболічна підтримка є частиною спортивної нутриціології, яка володіє як усіма рисами фундаментальної науки − термінами, поняттями, визначеннями, водночас має велику практичну значущість, допомагаючи як формувати актуальні і обґрунтовані програми нутритивно-метаболічної підтримки тренувального і змагального процесу спортсменів, так і сприяти збереженню здоров’я та якості життя спортсменів. Особливу важливість це положення знаходить в студентському спорті, де на організм спортсмена впливають додаткові стресові фактори, обумовлені складностями необхідності раціонального та ефективного поєднання навчального і тренувального процесу спортсменів високої кваліфікації. Нутритивно-метаболічні технології як методи метаболічної підтримки рухової активності повинні враховувати спеціалізацію і кваліфікацію спортсменів, їх статево-вікові особливості й застосовуватися з урахуванням періоду підготовки і спрямованості навантажень. У зв’язку з удосконаленням і посиленням допінг-контролю вкрай важливо, щоб продукти спортивного харчування та спеціальні харчові добавки, які широко застосовуються для оптимізації функціонального стану організму спортсмена підтримки його здоров’я та якості життя, не містили речовин, що належать до Забороненого списку WADA, й при цьому забезпечували виражений ергогенний ефект.
MATHEMATICAL MODELING OF THE STRESS-STRAIN STATE OF THE “BONE-IMPLANT” SYSTEM DURING THE OSTEOSYNTHESIS WITH A PHILOS WITH POLYLACTIC ACID IMPLANTS
(2020) Korzh, M. O.; Makarov, V. B.; Lipovsky, V. I.; Morozenko, D. V.; Danylchenko, S. I.; Данильченко, С. І.
The aim: To study was to use mathematical modeling in assessing the stress-strain state of the bone-implant system during plate osteosynthesis with a PHILOS plate of a proximal humerus fracture with polylactic acid implants. Materials and methods: Two bone-implant systems with a three-fragment humerus fracture according to the Neer classification (type 11-C1 according to the AO / ASIF classification) were selected for the study, one of which was with additional reinforcement of the head fragment with two polylactic acid implants (PLA – polylactide Ingeo™ Biopolymer 4032D). Sawbones (Europe AB, Malmö, Sweden) built the humeral model on 3D scanning of the composite model № 3404 of the left humerus. Results: A comparative analysis of the obtained results of mathematical modeling of the stress-strain state of the bone-implant systems showed that with given constraints (hand abduction to 90°), the use of two polylactic acid implants can reduce the stress in the plate and screws, respectively, by 11% and 6% . Conclusions: The use of polylactic acid implants during osteosynthesis of three- and four-fragment fractures of the proximal humerus, especially in the case of osteoporosis, allows providing for the reinforcement of metal structures and supporting of the articular surface without deterioration of fixation rigidity.
MODERN DIAGNOSTIC CRITERION FOR ESTABLISHING THE REGIONAL ORIGIN OF BLOOD IN SEXUAL CRIMES
(2022) Babkina, O. P.; Varukha, K. V.; Khmil, I. Y.; Danylchenko, S. I.; Ushko, Ia. A.; Данильченко, С. І.
The aim: Determination of regional blood origin in cases of sexual violence, establishing the possibility of using prostaglandin F2alpha as a marker of blood of menstrual origin. Materials and methods: The material for the study were samples of vaginal fluid, menstrual blood and capillary blood from females, the age distribution of women was carried out according to the gynecological classification according to the age periods of women’s lives depending on the functional state of their reproductive system: the first group – women at the age of 18-29, the second group – women at the age of 30-45. Results: Among objects of biological origin, fluids, especially blood, occupy an important place. The content of PGF2α has age fluctuations: its content is higher by 6-12% in women aged 30-45 than in women 18-29 years old. PGF2α levels above 13.1 ng / mg of dry tissue are a reliable sign of blood of menstrual origin, which is very important in determining the regional origin of blood in forensic examinations for sexual violence / sexual crimes. Conclusions: In cases of sexual violence against women, in addition to recording external harm, attention should also be paid to the examination of objects of biological origin, identification by species, sex, regional, organ or cell.
MORPHOMETRIC CHARACTERISTICS OF THE ORBIT IN ADULTS
(2023) Dubyna, S. O.; Serbin, S. I.; Bondarenko, S. V.; Danylchenko, S. І.; Fylenko, B. M.; Koptev, M. M.; Pyrog-Zakaznikova, A. V.; Дубина, С. О.; Сербин, С. И.; Бондаренко, С. В.; Данильченко, С. И.; Филенко, Б. Н.; Коптев, М. Н.; Пирог-Заказникова, А. В.; Данильченко, С. І.
The morphometric characteristics of the orbits of adults, in particular, left- and right-sided skewness, individual variability by age, gender and craniotypes were studied in order to obtain a morphometric standard of their anatomical norm. The study was conducted with the involvement of 96 Caucasian people aged 22 to 74 years (48 men, 48 women, average age 48.6 ± 3.2 years) without pathology of the craniofacial area. Our study was performed using such methods as computed tomography, morphometric method, and mathematical methods (variation and correlation analyses). According to the results of the study, it was found that there was no skewness of the orbits on the right and left, which made it possible to use the averaged indicators as a morphometric standard. The probable absence of gender dimorphism in the morphometric indicators of the orbits was noted. The presence of possible differences in the depth of the orbits and the length of the lower wall in the age group of the II period of mature age was established. Significant differences between individual morphometric indicators of the orbits in people with different craniotypes according to the cranial index were revealed. A comprehensive evaluation of morphometric indicators was carried out, which made it possible to establish such parameters as the quantitative norm of the length of the walls, the width and height of the entrance to the orbit, their depth, the angle of inclination of the entrance to the orbit and the angle between medial and lateral walls in adults in groups according to the right and left orbits, age, gender, and craniotypes. The quantitative norms of morphometric indicators of bone structures of the orbit were determined.
RELATIONSHIP BETWEEN PERIODONTAL DISEASES AND COVID-19
(2023) Vynogradova, O.; Dyryk, V.; Мinko, L.; Danylchenko, S.; Данильченко, С. І.
The problem of the high prevalence of inflammatory and dystrophic periodontal diseases is very urgent, despite the high level of diagnostic and treatment technologies in the arsenal of dentists and the availability of information for patients [1]. According to professional literature, the prevalence of perio- dontal tissue pathology is 85-100%, and their presence causes a number of biochemical, immunological and functional disorders, microcirculatory and metabolic disorders, disorders of almost all types of metabolism develop: protein, lipid, mineral, carbohydrate, which leads to to the progressive destruction of tissues of the oral cavity [2]. Such a high prevalence of periodontal diseases, as well as their close relation- ship with somatic pathology, determine the importance of this problem. The presence of concomitant pat- hology, in particular, cardiovascular, endocrinological, autoimmune and viral diseases is a significant factor that determines the course and prognosis of periodontal tissue diseases [3]. At the end of 2019, the world faced a terrible epidemic - COVID-19, which is still an acute scientific, medical and social problem. The complexity of the coronavirus disease caused by SARSCoV-2 lies in the unpredictable clinical course of the disease, which can progress rapidly, causing severe and fatal compli- cations . The high prevalence of this infection makes it necessary to study its pathogenetic mechanisms[4] in order to prescribe timely therapy. The relationship between underlying comorbidities and periodontal pathology is reasonably well understood, and the relationship between COVID-19 and periodontitis is in critical need of additional research.
THE CASES OF DOMESTIC VIOLENCE IN PROTRACTED QUARANTINE CAUSED BY COVID-19.
(2020) Вabkina, O. P.; Ushko, I. A.; Danylchenko, S. I.; Tarasyuk, V. O.; Vako, I. I.; Данильченко, С. І.
THE EFFECT OF PHYSICAL ACTIVITY ON THE SECRETORY FUNCTION OF MUSCLES AND BONE TISSUE IN HUMANS
(2023) Traverse, G.; Horoshko, V.; Danylchenko, S.; Траверсе, Г. М.; Горошко, В. І.; Данильченко, С. І.
The purpose of the study was to summarize new literature data on the effect of physical activity on the secretory function of skeletal muscles and their rela- tionship with organs. Materials and methods. Analytical methods were used in the study. The search for scientific information was carried out in databases of search systems. Results. Muscles secrete proteins called myok- ines, which are involved in a variety of processes by interacting with tissues. Current research has shown that exercise, by stimulating the skeletal muscle sys- tem in vivo, leads to the release of myokines and causes several effects that explain the positive effect of exercise in the treatment of several diseases of the musculoskeletal system. Skeletal muscles synthesize and secrete a wide range of myokines that contribute to various func- tions in organs, including the brain. Recent publica- tions have focused much attention on one such myo- kine, the recently discovered protein irisin, which is secreted into the bloodstream from skeletal muscle during exercise from the membrane-bound precursor fibronectin type III. Irisin promotes metabolic process- es such as glucose homeostasis and the darkening of white adipose tissue. Irisin also crosses the blood- brain barrier and initiates a neuroprotective genetic program in the hippocampus, which culminates in increased expression of brain-derived neurotrophic factors. Most studies report that irisin concentration is closely related to health status. For example, irisin levels are significantly lower in patients with obesity, osteoporosis/fracture, muscular atrophy, Alzheimer’s disease, and cardiovascular disease. Over the past decade, several myokines have been discovered, expanding our understanding of how muscles interact with other organs. In recent publications, a lot of attention is paid to the recently discovered protein irisin, which contributes to met- abolic processes. A series of studies on irisin have provided new insights into the mechanisms of exer- cise to improve bone density, counteract cartilage degeneration, and maintain overall joint environmen- tal homeostasis. These studies further contribute to the understanding of the role of exercise in the fight against osteoarthritis and may provide important assistance in advancing the prevention and treatment of this common disease. Research on the exercise-induced muscle factor irisin will help to better understand and explain the beneficial effects of exercise on maintaining physi- cal health, especially in the fight against aging and age-related degenerative changes. Conclusion. Thus, exercise-induced stimulation of bioactive cytokines increases muscle anabolism, bone formation, mitochondrial biogenesis, glucose utilization, and fatty acid oxidation, and reduces chronic inflammation. Метою дослідження було узагальнення нових літературних даних про вплив фізичної активності на секреторну функцію скелетних м’язів та її зв’язок з іншими органами. Матеріали і методи. У дослідженні використано аналітичний та бібліосемантичний методи. Пошук наукової інформації здійснювався в базах даних електронних пошукових систем. Результати. Сучасні дослідження показують, що фізичні вправи стимулюють скелетну мускулатуру in vivo, що призводить до вивільнення міокінів та спричиняє низку ефектів, які пояснюють позитивні ефекти фізичних вправ у лікуванні багатьох захворювань опорно-рухового апарату. Скелетні м’язи синтезують і виділяють різні міокіни, які сприяють різним функціям органів, включаючи мозок. Іризин, який був синтезований порівняно нещодавно, виділяється зі скелетних м’язів під час фізичних вправ. Іризин сприяє таким метаболічним процесам, як гомеостаз глюкози і потемніння білої жирової тканини. Іризин також долає гематоенцефалічний бар’єр і ініціює програму нейропротекторного гена в гіпокампі. Це збільшує експресію нейротрофічного фактора мозку. Більшість досліджень показали, що рівень іризину тісно пов’язаний зі станом здоров’я. Останні публікації приділили багато уваги нещодавно відкритому білку іризину, який сприяє метаболічним процесам. Серія досліджень щодо іризину дозволила по-новому зрозуміти механізми впливу на покращення щільності кісткової тканини, протидії дегенерації хряща та підтримки загального екологічного гомеостазу суглобів. Ці дослідження можуть додатково сприяти розумінню ролі фізичних вправ у боротьбі з остеоартритом і надати важливу підтримку в просуванні профілактики та його лікування. Висновок. Індукована фізичними вправами стимуляція біоактивних цитокінів збільшує анаболізм м’язів, формування кісток, мітохондріальний біогенез, утилізацію глюкози та окиснення жирних кислот, а також зменшує хронічне запалення. У перспективі розвитку дослідження іризину можуть краще пояснити сприятливий вплив фізичних вправ на підтримку фізичного здоров’я, особливо в боротьбі зі старінням і пов’язаними з віком дегенеративними змінами.