ІНДИВІДУАЛЬНІ КОЛЕКЦІЇ ВИКЛАДАЧІВ ТА СПІВРОБІТНИКІВ
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Item ЦИТОКІНІНДУКОВАНІ ЗМІНИ В ПОГЛИНАННІ ГЛЮКОЗИ ТА АКТИВНОСТІ ПІРУВАТКІНАЗИ В ОРГАНОТИПОВИХ КУЛЬТУРАХ(2025) Шкуропат, А. В.; Швець, В. А.; Головченко, І. В.; Гасюк, О. М.; Shkuropat, A. V.; Shvets, V. A.; Golovchenkо, I. V.; Hasiuk, O. M.Інтенсивність поглинання глюкози залежить від факторів, що впливають на її транспортери (GLUT). Вплив інтерлейкіну-2 (ІЛ-2) на поглинання глюкози суперечливий, а еритропоетин має антиапоптотичні властивості та знижує вміст глюкози. Мета нашого дослідження – з›ясувати вплив еритропоетину та ІЛ-2 на поглинання глюкози та активність піруваткінази М2 (PKM2) в органотипових культурах головного мозку, печінки та скелетних м›язів. Дослідження проводили на органотипових культурах, отриманих з печінки, головного мозку та скелетних м›язів білих лабораторних мишей. Культури інкубували з ІЛ-2 (5000, 7500 МО/мл), еритропоетином (13, 6,5 і 1,3 МО/мл) та без стимуляції (контроль). Органотипові культури печінки характеризувалися найнижчим рівнем поглинання глюкози. ІЛ-2 збільшував поглинання глюкози всіма культурами, а еритропоетин – тільки органотиповами культурами скелетних м›язів. Активність РКМ2 залежала від виду цитокінів: ІЛ-2 у низькій концентрації збільшував активність у органотипових культурах печінки та скелетних м›язів, еритропоетин у високій концентрації – знижував в органотипових культурах скелетних м›язів. Припускається, що еритропоетин активує GLUT4 у скелетних м›язах, а ІЛ-2 активує GLUT1 в усіх культурах, можливо, із залученням інших механізмів. Клітини, які збільшили активність PKM2 під впливом ІЛ-2, змінюють метаболізм з анаеробного на аеробний, а клітини зі зниженою активністю PKM2 під впливом еритропоетину, можливо, переключають метаболізм на гліколітичний шлях. Glucose uptake is regulated by the activity of glucose trans- porters (GLUT), which are influenced by various factors. The effects of interleukin-2 (IL-2) on glucose uptake remain con- troversial, while erythropoietin is known for its anti-apoptotic properties and glucose-lowering effects. This study aimed to evaluate the impact of IL-2 and erythropoietin on glucose up- take and pyruvate kinase M2 (PKM2) activity in organotypic cultures of brain, liver, and skeletal muscle. Organotypic cul- tures were prepared from white laboratory mice and incubated with IL-2 (5000 and 7500 IU/ml), erythropoietin (13, 6.5, and 1.3 IU/ml), or left untreated (control). Among all tissues, liver cultures exhibited the lowest glucose uptake. IL-2 increased glucose uptake in all culture types, while erythropoietin en- hanced uptake only in skeletal muscle cultures. PKM2 activity was cytokine- and tissue-dependent: low-dose IL-2 elevated PKM2 activity in liver and skeletal muscle cultures, whereas high-dose erythropoietin reduced PKM2 activity in skeletal muscle. These findings suggest that erythropoietin may activate GLUT4 in skeletal muscle, while IL-2 may act via GLUT1 across all tissue types, possibly involving other mechanisms. The observed increase in PKM2 activity under IL-2 stimula- tion may indicate a shift toward aerobic metabolism, while erythropoietin-induced decreases in PKM2 activity may reflect a shift toward glycolytic pathway.Item ФІЗИЧНА ТРЕНОВАНІСТЬ БІЛИХ МИШЕЙ ПІД ЧАС ПЛАВАННЯ В УМОВАХ СТИМУЛЯЦІЇ ТА ІНГІБУВАННЯ ІНТЕРЛЕЙКІНУ-2(2020) Швець, В. А.; Гасюк, О. М.; Shvets, V. A.; Hasiuk, O. M.Assessment of physical training is currently relevant, as it is known that the physical activity directly affects the cells of the immune system. Despite the sufficient quantity of studies on certain aspects of using IL-2 and its inhibitor, now there is a question about their dose-dependent effect on physical endurance. The purpose of the work was to determine the physical training of white mice in conditions of long-term administration of various doses of recombinant IL-2 and its inhibitor cyclosporine. Material and methods. 5 experimental groups of mice were formed (n=90). Group I received an inhibitor of IL-2 (10 mg/kg), II, III and IV experimental groups got IL-2 (5000, 7500 and 30000 IU/kg, respectively), group V received saline solution. Тhe method of forced swimming was used аs physical training to complete exhaustion with a load (7,5 % of body weight). The physical endurance was measured by the duration of swimming of mice from the moment of entry into the water until complete exhaustion. The study was divided into periods to determine adaptive changes (control, 1-4 and 6 week). Results and discussion. The animals with IL-2 inhibition throughout the experiment the swimming time increased: a significant veracious increase on the 2nd week was by 63±3 %; on the 4th week it increased by 44.4±2 %; on the 6th week it increased by 32±1.5 %. The swimming time of mice with exposure to IL-2 in the average concentration also increased throughout the training period, the largest veracious significant increase was on the 2nd (58.9±3 %), on the 3rd (20.9±1 %) and on the 6th (29.6±1.4 %) weeks. The studied indicator of the group of animals without the introduction of drugs reached the maximum on the 3rd week, then decreased slightly, but remained much higher than the initial, which can be considered the result of training. During the experiment, it was found out that in all experimental animals, the time of forced swimming during 6 weeks significantly exceeded the values obtained after the first day of the training. The maximum increase in physical training on the 6th week (compared to baseline) was observed in the group administered the IL-2 inhibitor (an increase at 267.4±13 %). Animals exposed to medium and high concentrations of IL-2 also showed a veracious increase of the studied indicator (at 161.65±8 % and 85.1±4 %, respectively). Conclusion. The obtained results showed that the inhibition of IL-2 had a positive effect on adaptation to physical training throughout the experiment and caused the most significant increase at the period of forced swimming compared to baseline. The introduction of IL-2 caused an increase in physical training of animals. However, only under the influence of IL-2 in the average concentration (7500 IU/kg), the swimming time increased in the post-action period, which indicated the adaptive effect of IL-2 in this concentration (which can be considered optimal).